pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A
(Plasmid
#74606)
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Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHR-mCherry-p2A
- Backbone size w/o insert (bp) 9757
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefull length human dynein light intermediate chain 1 Y101A, Y103A, W120A
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Alt nameLIC1
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SpeciesH. sapiens (human)
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Insert Size (bp)1599
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MutationY101A, Y103A, W120A
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Entrez GeneDYNC1LI1 (a.k.a. DLC-A, DNCLI1, LIC1)
- Promoter SFFV
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Tag
/ Fusion Protein
- HA tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer mCherry-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A was a gift from Ron Vale (Addgene plasmid # 74606 ; http://n2t.net/addgene:74606 ; RRID:Addgene_74606) -
For your References section:
A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. Schroeder CM, Ostrem JM, Hertz NT, Vale RD. Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351. 10.7554/eLife.03351 PubMed 25272277
