pHR-mCherry-p2A-LIC1 C-terminal half
(Plasmid #74603)

• Purpose: expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
• Depositing Lab: Ron Vale
• Publication: Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.

Backbone

• Vector backbone: pHR-mCherry-p2A
• Backbone size w/o insert (bp): 9757
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: full length human dynein light intermediate chain 1 amino acids 389-523
• Alt name: LIC1
• Species: H. sapiens (human)
• Insert Size (bp): 438
• Mutation: amino acids 389-523 of LIC1
• Entrez Gene: DYNC1LI1 (a.k.a. DLC-A, DNCLI1, LIC1)
• Promoter: SFFV
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: mCherry-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry