pGEX6P1-human LIC1 C-terminal half
(Plasmid #74599)

• Purpose: used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
• Depositing Lab: Ron Vale
• Publication: Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3) for protein production
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human dynein light intermediate chain 1 amino acids 389-523
• Alt name: LIC1
• Species: H. sapiens (human)
• Insert Size (bp): 408
• Mutation: amino acids 389-523 of LIC1
• Entrez Gene: DYNC1LI1 (a.k.a. DLC-A, DNCLI1, LIC1)
• Promoter: T7
• Tags / Fusion Proteins:
  • N-terminal GST (N terminal on backbone)
  • Strep II (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry