Phagemid Synuclein VHH Immune Library
(Pooled Library #1000000071)

• Purpose:

  Synuclein phagemid VHH phage display library. The depositors routinely use VCSM13 as helper phage, but other helper phage should be fine.

• Vector Backbone: JSC - GenBank: EU109715.1

• Depositing Labs: Anne Messer, Michael J Fox Foundation MJFF
• Publication: Mahajan et al. Sci Rep. 2018 Dec 4;8(1):17611. doi: 10.1038/s41598-018-35464-7.

Library Details

• Species: Camelid
• Vector Type: Phagemid

Bacteria Information

• Growth Temperature : 37 °C
• Resistance : Ampicillin
• Strain : TG1

For further manipulation, please use the growth strain/temperature recommended in your protocol as these will vary by method.

Library Shipping

This phage display library will be shipped frozen on dry ice.

• Volume: ∼100 µL aliquot of frozen bacterial glycerol stock (TG1 E. coli )

Resource Information

• Depositor Data:
  • Full Depositor Sequence (1)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Industry MTA

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

This phage display library will be provided as an aliquot of frozen bacterial glycerol stock (TG1 E. coli ) and will not be sequenced. For further manipulation, please use the growth stain/temperature recommended in your protocol as these will vary by method.

Library screening can be done by many approaches and may need to be tailored to the different targets and the reagents available. The depositor does not recommend amplification unless the primary library becomes unavailable for use in panning as amplification is known to create bias and reduce complexity. If amplification is required, or for general procedures, the depositor recommends following standard procedures such as: Barbas CF. (2001). Phage display: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

For general information, the depositor also recommends the following references:

Mukherjee, J., Tremblay, J. M., Leysath, C. E., Ofori, K., Baldwin, K., Feng, X., Bedenice, D., Webb, R. P., Wright, P. M., Smith, L. A., Tzipori, S., & Shoemaker, C. B. (2012). A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model. PloS One, 7(1), e29941. https://doi.org/10.1371/journal.pone.0029941 .

Maass, D. R., Sepulveda, J., Pernthaner, A., & Shoemaker, C. B. (2007). Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs). Journal of Immunological Methods, 324(1–2), 13–25. https://doi.org/10.1016/j.jim.2007.04.008 .

For more information about this library project, refer to the MJFF or MJFF Grant Funding web pages.