Alexandrov Cell-Free Protein Expression Kit (PEK)
(Kit #
1000000065
)
Depositing Lab: Kirill Alexandrov
This collection contains a set of 91 plasmids: 2 empty backbone control plasmids (pLTE EGFP and pCellFree_G03) and 89 open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov Küth FX. Biotechnol Bioeng 2015 Aug 24. doi: 10.1002/bit.25814. PubMed .
Description
The Alexandrov Cell-Free Protein Expression kit represents a set of 89 open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system. The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems. The set of proteins encoded is one of the most popular ones in the biomedical research community and range in size form 4 to 156 kDa, which enables the user to characterize the efficiency of their cell-free system in correlation to the size of the product. The analysis can be conveniently performed using the N-terminal eGFP tag carried by all constructs.
Please note that Well-G6 is a duplicate of Well-G5. Both Wells contain plasmid pCellFree_G03 STAT3.
Kit Documentation
Documents included with shipment: alexandrov-cell-free-PEK.pdf 76.3 KB
Additional Reference
The pCellFree_G03 plasmid provided with this kit was generated in a different publication. Please, cite the following reference when using this plasmid:
Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems. Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Škalamera D, Gonda TJ, Alexandrov K. J Biotechnol 2015 Feb 10;195:1-7. doi: 10.1016/j.jbiotec.2014.12.006. PubMed .
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Alexandrov Cell-Free Protein Expression Kit was a gift from Kirill Alexandrov (Addgene kit # 1000000065).”
For your Reference section:
Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov Küth FX. Biotechnol Bioeng 2015 Aug 24. doi: 10.1002/bit.25814. PubMed .
Alexandrov Cell-Free Protein Expression Kit (PEK) - #1000000065
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Chloramphenicol and Ampicillin |