Multiple Lentiviral Expression System Kit
(Kit #
1000000060
)
Depositing Lab: Ian Frew
The Multiple Lentiviral Expression (MuLE) is a modular and flexible system that can be used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
This kit contains 96 plasmids and is shipped as bacterial glycerol stocks in a 96-well plate format.
Original Publication
A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Gonçalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Apr 1;125(4):1603-19. doi: 10.1172/JCI79743. Epub 2015 Mar 9. PubMed .
Description
The MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic alterations into mammalian cells. The MuLE system kit consists of 91 building block Entry vectors and 5 lentiviral Destination vectors to allow simple and rapid MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses containing up to five different genetic expression elements. These vectors can induce combinatorial constitutive or inducible gene overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies. The ability to simultaneously alter multiple genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic modulation studies in mammalian cells and tissues.
Overview of MuLE vector assembly
Schematic overview of a recombination reaction involving four MuLE Entry vectors and a lentiviral Destination vector, allowing combinatorial expression of combinations of the listed genetic elements. A series of vectors with similar genetic elements are also available to allow recombination reactions involving two or three Entry vectors
Kit Documentation
Documents included with shipment:
Multiple Lentiviral Expression System Kit 79.1 KBResources
Download a zip file of MuLE vector sequences in pDRAW32 format or plain text. Please note that the five pMuLE ENTR U6-miR-30 vectors (IDs 62116-62120) do not have a full sequence available.
MuLE vector sequences pDRAW32 241.7 KB MuLE vector sequences text files 140.9 KB
Additional Entry vectors compatible with the MuLE system kit. These plasmids are not included in the kit and must be ordered separately:
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The MuLE system kit was a gift from Ian Frew (Addgene kit # 1000000060).”
For your Reference section:
A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Gonçalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Apr 1;125(4):1603-19. doi: 10.1172/JCI79743. Epub 2015 Mar 9. PubMed .
Multiple Lentiviral Expression (MuLE) system - #1000000060
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Kanamycin | |
| Chloramphenicol and Ampicillin |