Golden Gate TALEN Accessory Pack
(Kit #
1000000030
)
Depositing Lab: Takashi Yamamoto
This supplemental package contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.
This kit consists of 9 individually packaged plasmids, which are shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Please note that the Golden Gate TALEN and TAL Effector Kit is required to use the plasmids in this accessory pack.
Original Publication
Efficient TALEN construction and evaluation methods for human cell and animal applications. Sakuma T, Hosoi S, Woltjen K, Suzuki K, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T. Genes Cells. 2013 Apr;18(4):315-26. doi: 10.1111/gtc.12037. Epub 2013 Feb 6. PubMed PMID 23388034.
Description
This supplemental package contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains (N153AA, C47AA).
Protocols
Protocol for TALEN construction and evaluation as described in the article.
Yamamoto protocol 306.0 KBHow to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The plasmid kit used for building TALENs and TALE-TFs was a gift from Takashi Yamamoto (Addgene kit #1000000030)"
Efficient TALEN construction and evaluation methods for human cell and animal applications. Sakuma T, Hosoi S, Woltjen K, Suzuki K, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T. Genes Cells. 2013 Apr;18(4):315-26. doi: 10.1111/gtc.12037. Epub 2013 Feb 6. PubMed PMID 23388034.