Open Source Wnt Project Plasmids
(Kit #
1000000022
)
Depositing Labs:
Marian Waterman,
David Virshup
The Open Source Wnt Kit is a 95-plasmid library of all 19 Wnt clones. The collection contains full-length open reading frames (ORFs) for every human Wnt ligand.
This kit consists of 1 plate, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Original Publication
A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation . 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PubMed .
Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J. Biol. Chem. . 2014 May 19; doi:10.1074/jbc.M114.575027 PubMed .
Description
The Open Source Wnt Project was initiated to create a collection of standardized Wnt plasmids that are easily accessible to the research community. The project and plasmids were created by Dr. Marian Waterman's group at UC Irvine and Dr. David Virshup's group at Duke Singapore as a way to pool community resources and efforts to develop an accessible and affordable set of reagents for the Wnt community. The Open Source Wnt Kit is a 95-plasmid library of all 19 Wnt clones. The collection contains full-length open reading frames (ORFs) for every human Wnt ligand.
In addition to this original set of 76 clones, Dr. Bryan MacDonald and Dr. Xi He have contributed a modified version of each tagged Wnt that enables epitope tagging without loss of Wnt signaling activity.
Read Addgene's Septemeber 2013 interview with Marian Waterman, Crowd-Sourced Science: The Open Source Wnt Project .
Kit Documentation
Information and Plate Map PDF 96.8Â KBResources
Open Source Wnt Info Sheet 94.4Â KB
Open source Wnt Nomenclature 42.5Â KB
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The plasmid kit used in this publication was a gift from Marian Waterman, David Virshup and Xi He (Addgene kit # 1000000022)"
For your Reference section:
A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation . 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PubMed .
Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J. Biol. Chem. . 2014 May 19; doi:10.1074/jbc.M114.575027 PubMed .
Wnt Open Source - #1000000022
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Kanamycin | |
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | Wnt1 STOP | Kanamycin |
| A / 2 | Wnt2 STOP | Kanamycin |
| A / 3 | Wnt2B2 STOP | Kanamycin |
| A / 4 | Wnt3A STOP | Kanamycin |
| A / 5 | Wnt3 STOP | Kanamycin |
| A / 6 | Wnt4 STOP | Kanamycin |
| A / 7 | Wnt5A STOP | Kanamycin |
| A / 8 | Wnt5B STOP | Kanamycin |
| A / 9 | Wnt6 STOP | Kanamycin |
| A / 10 | Wnt7A STOP | Kanamycin |
| A / 11 | Wnt7B STOP | Kanamycin |
| A / 12 | Wnt8A STOP | Kanamycin |
| B / 1 | Wnt8B STOP | Kanamycin |
| B / 2 | Wnt9A STOP | Kanamycin |
| B / 3 | Wnt9B STOP | Kanamycin |
| B / 4 | Wnt10A STOP | Kanamycin |
| B / 5 | Wnt10B STOP | Kanamycin |
| B / 6 | Wnt11 STOP | Kanamycin |
| B / 7 | Wnt16 STOP | Kanamycin |
| B / 8 | Wnt1 Non-STOP | Kanamycin |
| B / 9 | Wnt2 Non-STOP | Kanamycin |
| B / 10 | Wnt2B2 Non-STOP | Kanamycin |
| B / 11 | Wnt3A Non-STOP | Kanamycin |
| B / 12 | Wnt3 Non-STOP | Kanamycin |
| C / 1 | Wnt4 Non-STOP | Kanamycin |
| C / 2 | Wnt5A Non-STOP | Kanamycin |
| C / 3 | Wnt5B Non-STOP | Kanamycin |
| C / 4 | Wnt6 Non-STOP | Kanamycin |
| C / 5 | Wnt7A Non-STOP | Kanamycin |
| C / 6 | Wnt7B Non-STOP | Kanamycin |
| C / 7 | Wnt8A Non-STOP | Kanamycin |
| C / 8 | Wnt8B Non-STOP | Kanamycin |
| C / 9 | Wnt9A Non-STOP | Kanamycin |
| C / 10 | Wnt9B Non-STOP | Kanamycin |
| C / 11 | Wnt10A Non-STOP | Kanamycin |
| C / 12 | Wnt11 Non-STOP | Kanamycin |
| D / 1 | Wnt16 Non-STOP | Kanamycin |
| D / 2 | Wnt10B Non-STOP | Kanamycin |
| D / 3 | pcDNA-Wnt2 | Ampicillin |
| D / 4 | pcDNA-Wnt2B2 | Ampicillin |
| D / 5 | pcDNA-Wnt3A | Ampicillin |
| D / 6 | pcDNA-Wnt3 | Ampicillin |
| D / 7 | pcDNA-Wnt4 | Ampicillin |
| D / 8 | pcDNA-Wnt5A | Ampicillin |
| D / 9 | pcDNA-Wnt5B | Ampicillin |
| D / 10 | pcDNA-Wnt6 | Ampicillin |
| D / 11 | pcDNA-Wnt7A | Ampicillin |
| D / 12 | pcDNA-Wnt7B | Ampicillin |
| E / 1 | pcDNA-Wnt8A | Ampicillin |
| E / 2 | pcDNA-Wnt8B | Ampicillin |
| E / 3 | pcDNA-Wnt9A | Ampicillin |
| E / 4 | pcDNA-Wnt9B | Ampicillin |
| E / 5 | pcDNA-Wnt10A | Ampicillin |
| E / 6 | pcDNA-Wnt10B | Ampicillin |
| E / 7 | pcDNA-Wnt11 | Ampicillin |
| E / 8 | pcDNA-Wnt16 | Ampicillin |
| E / 9 | pcDNA-Wnt1-V5 | Ampicillin |
| E / 10 | pcDNA-Wnt2-V5 | Ampicillin |
| E / 11 | pcDNA-Wnt2B2-V5 | Ampicillin |
| E / 12 | pcDNA-Wnt3A-V5 | Ampicillin |
| F / 1 | pcDNA-Wnt3-V5 | Ampicillin |
| F / 2 | pcDNA-Wnt4-V5 | Ampicillin |
| F / 3 | pcDNA-Wnt5A-V5 | Ampicillin |
| F / 4 | pcDNA-Wnt5B-V5 | Ampicillin |
| F / 5 | pcDNA-Wnt6-V5 | Ampicillin |
| F / 6 | pcDNA-Wnt7A-V5 | Ampicillin |
| F / 7 | pcDNA-Wnt7B-V5 | Ampicillin |
| F / 8 | pcDNA-Wnt8A-V5 | Ampicillin |
| F / 9 | pcDNA-Wnt8B-V5 | Ampicillin |
| F / 10 | pcDNA-Wnt9A-V5 | Ampicillin |
| F / 11 | pcDNA-Wnt9B-V5 | Ampicillin |
| F / 12 | pcDNA-Wnt10A-V5 | Ampicillin |
| G / 1 | pcDNA-Wnt10B-V5 | Ampicillin |
| G / 2 | pcDNA-Wnt11-V5 | Ampicillin |
| G / 3 | pcDNA-Wnt16-V5 | Ampicillin |
| G / 4 | pcDNA-Wnt1 | Ampicillin |
| G / 5 | Active Wnt1-V5 | Ampicillin |
| G / 6 | Active Wnt2B-V5 | Ampicillin |
| G / 7 | Active Wnt2-V5 | Ampicillin |
| G / 8 | Active Wnt3A-V5 | Ampicillin |
| G / 9 | Active Wnt3-V5 | Ampicillin |
| G / 10 | Active Wnt4-V5 | Ampicillin |
| G / 11 | Active Wnt5A-V5 | Ampicillin |
| G / 12 | Active Wnt5B-V5 | Ampicillin |
| H / 1 | Active Wnt6-V5 | Ampicillin |
| H / 2 | Active Wnt7A-V5 | Ampicillin |
| H / 3 | Active Wnt7B-V5 | Ampicillin |
| H / 4 | Active Wnt8A-V5 | Ampicillin |
| H / 5 | Active Wnt8B-V5 | Ampicillin |
| H / 6 | Active Wnt9A-V5 | Ampicillin |
| H / 7 | Active Wnt9B-V5 | Ampicillin |
| H / 8 | Active Wnt10A-V5 | Ampicillin |
| H / 9 | Active Wnt10B-V5 | Ampicillin |
| H / 10 | Active Wnt11-V5 | Ampicillin |
| H / 11 | Active Wnt16-V5 | Ampicillin |