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pcFLAG-Grap2-R83E
(Plasmid #74512)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcFLAG
  • Backbone manufacturer
    this lab, modified from pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grap2
  • Species
    H. sapiens (human)
  • Mutation
    R83E
  • Entrez Gene
    GRAP2 (a.k.a. GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, P38)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor notes that a BamHI restriction site was inserted following the start codon of Grap2. This site does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcFLAG-Grap2-R83E was a gift from Lars Rönnstrand (Addgene plasmid # 74512 ; http://n2t.net/addgene:74512 ; RRID:Addgene_74512)

  • For your References section:

    Expression of GADS enhances FLT3-induced mitogenic signaling. Chougule RA, Cordero E, Moharram SA, Pietras K, Ronnstrand L, Kazi JU. Oncotarget. 2016 Feb 15. doi: 10.18632/oncotarget.7415. 10.18632/oncotarget.7415 PubMed 26895103
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