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pMSCVneo-FYN-WT-Myc-FLAG
(Plasmid #74510)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FYN
  • Species
    H. sapiens (human)
  • Entrez Gene
    FYN (a.k.a. SLK, SYN, p59-FYN)
  • Promoter unknown
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pLXSN5'
  • 3′ sequencing primer MSCV_C_rev2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor notes that a BamHI restriction site was inserted following the start codon of FYN. This site does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVneo-FYN-WT-Myc-FLAG was a gift from Lars Rönnstrand (Addgene plasmid # 74510 ; http://n2t.net/addgene:74510 ; RRID:Addgene_74510)

  • For your References section:

    FYN expression potentiates FLT3-ITD induced STAT5 signaling in acute myeloid leukemia. Chougule RA, Kazi JU, Ronnstrand L. Oncotarget. 2016 Feb 2. doi: 10.18632/oncotarget.7128. 10.18632/oncotarget.7128 PubMed 26848862
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