pcFLAG-FYN-WT
(Plasmid #74509)

• Purpose: Overexpression of FYN in mammalian cells
• Depositing Lab: Lars Rönnstrand
• Publication: Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/oncotarget.7128.

Backbone

• Vector backbone: pcFLAG
• Backbone manufacturer: this lab, modified from pcDNA3
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FYN
• Species: H. sapiens (human)
• Entrez Gene: FYN (a.k.a. SLK, SYN, p59-FYN)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor notes that a BamHI restriction site was inserted following the start codon of FYN. This site does not affect plasmid function.