pMSCVneo-HOXB3-FLAG
(Plasmid
#74508)
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PurposeRetroviral expression of HOXB3
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB3
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SpeciesH. sapiens (human)
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Entrez GeneHOXB3 (a.k.a. HOX2, HOX2G, Hox-2.7)
- Promoter unknown
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Tag
/ Fusion Protein
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer MSCV_C_rev2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVneo-HOXB3-FLAG was a gift from Lars Rönnstrand (Addgene plasmid # 74508 ; http://n2t.net/addgene:74508 ; RRID:Addgene_74508) -
For your References section:
The role of HOXB2 and HOXB3 in acute myeloid leukemia. Lindblad O, Chougule RA, Moharram SA, Kabir NN, Sun J, Kazi JU, Ronnstrand L. Biochem Biophys Res Commun. 2015 Nov 27;467(4):742-7. doi: 10.1016/j.bbrc.2015.10.071. Epub 2015 Oct 22. 10.1016/j.bbrc.2015.10.071 PubMed 26482852