pMSCVBEX1-WT-Myc-FLAG
(Plasmid #74506)

• Purpose: Retroviral expression of BEX1
• Depositing Lab: Lars Rönnstrand
• Publication: Lindblad et al Oncotarget. 2015 Aug 28;6(25):21395-405.

Backbone

• Vector backbone: pMSCV
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BEX1
• Species: H. sapiens (human)
• Entrez Gene: BEX1 (a.k.a. BEX2, HBEX2, HGR74-h)
• Promoter: unknown
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pLXSN5'
• 3′ sequencing primer: MSCV_C_rev2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry