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Addgene

pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)
(Plasmid #74505)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcFLAG
  • Backbone manufacturer
    this lab, modified from pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CSK
  • Species
    H. sapiens (human)
  • Mutation
    SH3-SH2 domains
  • Entrez Gene
    CSK
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids) was a gift from Lars Rönnstrand (Addgene plasmid # 74505 ; http://n2t.net/addgene:74505 ; RRID:Addgene_74505)

  • For your References section:

    The tyrosine kinase CSK associates with FLT3 and c-Kit receptors and regulates downstream signaling. Kazi JU, Vaapil M, Agarwal S, Bracco E, Pahlman S, Ronnstrand L. Cell Signal. 2013 Sep;25(9):1852-60. doi: 10.1016/j.cellsig.2013.05.016. Epub 2013 May 21. 10.1016/j.cellsig.2013.05.016 PubMed 23707526
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