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Addgene

pcDNA3-SLAP-FLAG
(Plasmid #74500)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74500 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLAP
  • Species
    H. sapiens (human)
  • Mutation
    N18K, G74A, and T149A
  • Entrez Gene
    SLA (a.k.a. SLA1, SLAP)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-SLAP-FLAG was a gift from Lars Rönnstrand (Addgene plasmid # 74500 ; http://n2t.net/addgene:74500 ; RRID:Addgene_74500)

  • For your References section:

    Src-Like adaptor protein (SLAP) binds to the receptor tyrosine kinase Flt3 and modulates receptor stability and downstream signaling. Kazi JU, Ronnstrand L. PLoS One. 2012;7(12):e53509. doi: 10.1371/journal.pone.0053509. Epub 2012 Dec 31. 10.1371/journal.pone.0053509 PubMed 23300935
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