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pMSCV-Flt3-ITD-Y591F/Y919F
(Plasmid #74499)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLT3
  • Species
    H. sapiens (human)
  • Mutation
    Y591F/Y919F, T227M
  • Entrez Gene
    FLT3 (a.k.a. CD135, FLK-2, FLK2, STK1)
  • Promoter unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pLXSN5'
  • 3′ sequencing primer MSCV_C_rev2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 287, NO. 43, pp. 36509_36517, October 19, 2012

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-Flt3-ITD-Y591F/Y919F was a gift from Lars Rönnstrand (Addgene plasmid # 74499 ; http://n2t.net/addgene:74499 ; RRID:Addgene_74499)

  • For your References section:

    Suppressor of cytokine signaling 6 (SOCS6) negatively regulates Flt3 signal transduction through direct binding to phosphorylated tyrosines 591 and 919 of Flt3. Kazi JU, Sun J, Phung B, Zadjali F, Flores-Morales A, Ronnstrand L. J Biol Chem. 2012 Oct 19;287(43):36509-17. doi: 10.1074/jbc.M112.376111. Epub 2012 Sep 5. 10.1074/jbc.M112.376111 PubMed 22952242
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