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Addgene

pCas9(D10A)
(Plasmid #74495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWTCas9
  • Backbone manufacturer
    Derived from Addgene #44250 (http://www.cell.com/abstract/S0092-8674(13)00211-0)
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 D10A
  • Alt name
    Nicking Cas9
  • Species
    Synthetic
  • Mutation
    D10A
  • Promoter pTeto

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Addgene #44250 (See: http://www.cell.com/abstract/S0092-8674(13)00211-0)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9(D10A) was a gift from Xiao Wang (Addgene plasmid # 74495 ; http://n2t.net/addgene:74495 ; RRID:Addgene_74495)

  • For your References section:

    Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases. Standage-Beier K, Zhang Q, Wang X. ACS Synth Biol. 2015 Nov 20;4(11):1217-25. doi: 10.1021/acssynbio.5b00132. Epub 2015 Oct 25. 10.1021/acssynbio.5b00132 PubMed 26451892
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