pJA291
(Plasmid #74487)

• Purpose: eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP
• Depositing Lab: Andrew Fire
• Publication: Arribere et al Nature. 2016 Jun 1;534(7609):719-723. doi: 10.1038/nature18308.

Backbone

• Vector backbone: pMCB306
• Backbone manufacturer: Bassik Lab
• Backbone size w/o insert (bp): 7167
• Total vector size (bp): 9167
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: puroR-mCherry-T2A-eGFP
• Species: Synthetic
• Insert Size (bp): 2000
• Promoter: EF1alpha

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: nextera
• 3′ sequencing primer: nextera

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry