pJA327
(Plasmid
#74486)
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Purposesuperfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneL3785
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Backbone manufacturerFire Lab
- Backbone size w/o insert (bp) 5419
- Total vector size (bp) 6419
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesuperfolder GFP
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SpeciesSynthetic
- Promoter myo-3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer AF-KLA-20
- 3′ sequencing primer AF-ZF-337 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJA327 was a gift from Andrew Fire (Addgene plasmid # 74486 ; http://n2t.net/addgene:74486 ; RRID:Addgene_74486) -
For your References section:
Translation readthrough mitigation. Arribere JA, Cenik ES, Jain N, Hess GT, Lee CH, Bassik MC, Fire AZ. Nature. 2016 Jun 1;534(7609):719-723. doi: 10.1038/nature18308. 10.1038/nature18308 PubMed 27281202
Map uploaded by the depositor.
