pCMV-myc-FRNK
(Plasmid #74481)

• Purpose: This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).
• Depositing Lab: J Thomas Parsons
• Publication: Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

Backbone

• Vector backbone: pCMV-myc
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FRNK
• Species: G. gallus (chicken)
• Mutation: FRNK is the C-terminal, noncatalytic domain of the FAK kinase
• Entrez Gene: PTK2 (a.k.a. FAK, FAK1, FRNK, p125FAK)
• Promoter: CMV
• Tag / Fusion Protein:
  • c-myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry