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Addgene

pBS-FAK
(Plasmid #74478)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74478 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-KS-
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FAK
  • Species
    G. gallus (chicken)
  • Entrez Gene
    PTK2 (a.k.a. FAK, FAK1, FRNK, p125FAK)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-FAK was a gift from J Thomas Parsons (Addgene plasmid # 74478 ; http://n2t.net/addgene:74478 ; RRID:Addgene_74478)

  • For your References section:

    pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5192-6. PubMed 1594631
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