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Addgene

pCR3.1-mIL2
(Plasmid #74467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5060
  • Total vector size (bp) 5635
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL2
  • Alt name
    interleukin 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    575
  • Entrez Gene
    Il2 (a.k.a. Il-2)
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from plasmid pCDmouseIL2, C/N 39892, ATCC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3.1-mIL2 was a gift from Loree Heller (Addgene plasmid # 74467 ; http://n2t.net/addgene:74467 ; RRID:Addgene_74467)

  • For your References section:

    In vivo electroporation of plasmids encoding GM-CSF or interleukin-2 into existing B16 melanomas combined with electrochemotherapy induces long-term antitumour immunity. Heller L, Pottinger C, Jaroszeski MJ, Gilbert R, Heller R. Melanoma Res. 2000 Dec;10(6):577-83. PubMed 11198480
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