pCR3.1-mGM-CSF
(Plasmid #74465)

• Purpose: expression of mGM-CSF in mammalian cells
• Depositing Lab: Loree Heller
• Publication: Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Backbone

• Vector backbone: pCR3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5060
• Total vector size (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: granulocyte-monocyte colony-stimulating factor
• Alt name: Csf2
• Species: M. musculus (mouse)
• Insert Size (bp): 442
• GenBank ID: NM_009969
• Entrez Gene: Csf2 (a.k.a. CSF, Csfgm, GMCSF, Gm-CSf, MGI-IGM)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was derived by PCR and T/A cloning.