Aiolos ZF2 (AA146-168) in Cilantro 2
(Plasmid
#74456)
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PurposeMonitor post-translational degradation of Aiolos ZF2 via EGFP:mCherry ratio (Lentiviral, PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLentiviral
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Backbone manufacturerBroad Institute
- Backbone size w/o insert (bp) 10603
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAiolos (IKZF3) AA130-189
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SpeciesH. sapiens (human)
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Insert Size (bp)69
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Entrez GeneIKZF3 (a.k.a. AIO, AIOLOS, IMD84, ZNFN1A3)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer hPGK-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Aiolos ZF2 (AA146-168) in Cilantro 2 was a gift from Benjamin Ebert (Addgene plasmid # 74456 ; http://n2t.net/addgene:74456 ; RRID:Addgene_74456)
