Aiolos in Artichoke
(Plasmid #74452)

• Purpose: Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
• Depositing Lab: Benjamin Ebert
• Publication: Artichoke (unpublished)

Backbone

• Vector backbone: Lentiviral
• Backbone manufacturer: Genetic Perturbations Platform, Broad Institute
• Backbone size w/o insert (bp): 10553
• Total vector size (bp): 12077
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Aiolos (IKZF3)
• Species: H. sapiens (human)
• Insert Size (bp): 1524
• Entrez Gene: IKZF3 (a.k.a. AIO, AIOLOS, IMD84, ZNFN1A3)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SFFVpro-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • Aiolos (IKZF3) in Artichoke (SFFV.GFP.IRES.mCherry.cppt.EF1a.PuroR)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry