Aiolos AA130-189 in Artichoke
(Plasmid
#74451)
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PurposeMonitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLentiviral
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Backbone manufacturerGenetic Perturbations Platform, Broad Institute
- Backbone size w/o insert (bp) 10553
- Total vector size (bp) 10745
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAiolos (IKZF3) AA130-189
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SpeciesH. sapiens (human)
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Insert Size (bp)1524
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Entrez GeneIKZF3 (a.k.a. AIO, AIOLOS, IMD84, ZNFN1A3)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SFFVpro-F
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Aiolos AA130-189 in Artichoke was a gift from Benjamin Ebert (Addgene plasmid # 74451 ; http://n2t.net/addgene:74451 ; RRID:Addgene_74451)
