pUC57-mitoLbNOX
(Plasmid #74448)

• Purpose: This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
• Depositing Lab: Vamsi Mootha
• Publication: Titov et al Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. Epub 2016 Apr 7.

Backbone

• Vector backbone: PUC57
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 2850

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitoLbNOX
• Alt name: H2O-forming NADH oxidase from Lactobacillus brevis
• Species: Lactobacillus brevis
• Insert Size (bp): 1490
• Mutation: Human codon optimized
• Tags / Fusion Proteins:
  • Flag tag with linker (C terminal on insert)
  • mitochondrial targeting sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Mlu1 (not destroyed)
• 5′ sequencing primer: GTAAAACGACGGCCAGTG
• 3′ sequencing primer: GGAAACAGCTATGACCATG

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In our original publication we express mitoLbNOX in mammalian cells using a Tet3G dox-inducible system from Clontech. We cannot distribute those exact plasmids due to Clontech license restrictions. We recommend that investigators subclone this mitoLbNOX sequence into their preferred expression system.