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Addgene

pLenti.PGK.blast-Flag-MFF-S155,172D
(Plasmid #74442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLENTI PGK Blast DEST (w524-1)
  • Backbone manufacturer
    Addgene plasmid 19065
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flag-MFF
  • Alt name
    Q9GZY8-5
  • Species
    H. sapiens (human)
  • Mutation
    Ser 155 Asp, Ser 172 Asp (Numbering based on MFF isoform 1)
  • Entrez Gene
    MFF (a.k.a. C2orf33, EMPF2, GL004)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti.PGK.blast-Flag-MFF-S155,172D was a gift from Reuben Shaw (Addgene plasmid # 74442 ; http://n2t.net/addgene:74442 ; RRID:Addgene_74442)

  • For your References section:

    Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, Young NP, Chen H, Polleux F, Chan DC, Shaw RJ. Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138. 10.1126/science.aab4138 PubMed 26816379
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