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Addgene

pCDNA3-STAT3-Y705F
(Plasmid #74434)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74434 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
  • Species
    M. musculus (mouse)
  • Mutation
    Mutations E16K, Y705F
  • GenBank ID
    NP_998824.1
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E16K mutation originated in Plasmid #8706 but does not affect function.

WT control for Plasmid 74434: pCDNA3-STAT3-Y705F is Plasmid 8706: Stat3 pcDNA3 from the Darnell lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-STAT3-Y705F was a gift from Jie Chen (Addgene plasmid # 74434 ; http://n2t.net/addgene:74434 ; RRID:Addgene_74434)

  • For your References section:

    Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation. Kim JH, Yoon MS, Chen J. J Biol Chem. 2009 Dec 18;284(51):35425-32. doi: 10.1074/jbc.M109.051516. Epub . 10.1074/jbc.M109.051516 PubMed 19875458
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