pmirGlo-CALB2-3'UTR delta ARE&mir30
(Plasmid
#74430)
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PurposeCalb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmirGLO
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 7350
- Total vector size (bp) 7893
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCALB2 3'UTR
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SpeciesH. sapiens (human)
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Insert Size (bp)570
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GenBank IDNM_001740
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Entrez GeneCALB2 (a.k.a. CAB29, CAL2, CR)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer mPGK-F
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmirGlo-CALB2-3'UTR delta ARE&mir30 was a gift from Emanuela Felley-Bosco & Rolf Stahel (Addgene plasmid # 74430 ; http://n2t.net/addgene:74430 ; RRID:Addgene_74430) -
For your References section:
Posttranscriptional Regulation Controls Calretinin Expression in Malignant Pleural Mesothelioma. Kresoja-Rakic J, Sulemani M, Kirschner MB, Ronner M, Reid G, Kao S, Schwaller B, Weder W, Stahel RA, Felley-Bosco E. Front Genet. 2017 May 29;8:70. doi: 10.3389/fgene.2017.00070. eCollection 2017. 10.3389/fgene.2017.00070 PubMed 28611824