pCMV-GCaMP3bright
(Plasmid #74423)

• Purpose: Express fluorescent calcium indicator GCaMP3bright in mammalian cells
• Depositing Lab: Katalin Torok
• Publication: Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.1038/srep15978.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3945
• Total vector size (bp): 5308
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP3bright
• Alt name: GCaMP3 EF-4
• Species: R. norvegicus (rat); A. victoria (jellyfish)
• Insert Size (bp): 1363
• Mutation: D392A
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A portion of this plasmid was derived from a plasmid made by Loren L. Looger

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry