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Addgene

pRFP.mIKKε
(Plasmid #74414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRFP.C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse IKKε
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ikbke (a.k.a. IKK-E, IKK-i, IKKepsilon, Ikki)
  • Promoter CMV
  • Tag / Fusion Protein
    • RFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer gaacgcgccgagggccgccactc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRFP.mIKKε was a gift from Georgios Stathopoulos (Addgene plasmid # 74414 ; http://n2t.net/addgene:74414 ; RRID:Addgene_74414)
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