FLAGHis10pFastBacHTA/Serca1
(Plasmid
#74401)
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Purposeexpression of SERCA in insect cells
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBacHTA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4870
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSERCA1
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Speciesrabbit
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Insert Size (bp)3003
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MutationNcoI site destroyed (silent) T898
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GenBank IDNM_001089318.1
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Entrez GeneATP2A1 (a.k.a. ATP2A3, SERCA1a)
- Promoter polyhedrin
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Tags
/ Fusion Proteins
- FLAG (N terminal on backbone)
- 10His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Polyhedrin-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
FLAG tag followed by 4His added upstream of original N-terminal His tag
KpnI and HindIII sites were destroyed by silent mutations in pFastBacHTA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAGHis10pFastBacHTA/Serca1 was a gift from Robert Nakamoto (Addgene plasmid # 74401 ; http://n2t.net/addgene:74401 ; RRID:Addgene_74401)