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pFastBacI/MDR3cysless
(Plasmid #74400)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBacI
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MDR3
  • Alt name
    Pgp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3864
  • Mutation
    all Cys to Ala
  • GenBank ID
  • Entrez Gene
    Abcb1a (a.k.a. Abcb4, Evi32, Mdr1a, Mdr3, P-gp, Pgp, Pgy-3, Pgy3, mdr-3)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 10His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer Polyhedrin-F
  • 3′ sequencing primer pFastBac-Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Hassane Mchaourab, Vanderbilt University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBacI/MDR3cysless was a gift from Robert Nakamoto (Addgene plasmid # 74400 ; http://n2t.net/addgene:74400 ; RRID:Addgene_74400)
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