pISH-Neurog1
(Plasmid
#74395)
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PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameneurogenin 1
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Alt nameNeurog1
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Alt nameNgn1
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SpeciesM. musculus (mouse)
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Insert Size (bp)701
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GenBank IDNM_010896.2
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Entrez GeneNeurog1 (a.k.a. AKA, Math4C, Neurod3, bHLHa6, ngn1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SpeI digestion and T7 RNA polymerase for antisense probe
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Neurog1 was a gift from Peter Mombaerts (Addgene plasmid # 74395 ; http://n2t.net/addgene:74395 ; RRID:Addgene_74395) -
For your References section:
Expression patterns of homeobox genes in the mouse vomeronasal organ at postnatal stages. Chang I, Parrilla M. Gene Expr Patterns. 2016 Jul;21(2):69-80. doi: 10.1016/j.gep.2016.08.001. Epub 2016 Aug 9. 10.1016/j.gep.2016.08.001 PubMed 27521061