pISH-Satb1
(Plasmid #74357)

• Purpose: Use to synthesize riboprobes for in situ hybridization (ISH) probe for Satb1. Not for gene expression.
• Depositing Lab: Peter Mombaerts
• Publication: Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19.

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: special AT-rich sequence binding protein 1
• Alt name: Satb1
• Species: M. musculus (mouse)
• Insert Size (bp): 932
• Entrez Gene: Satb1 (a.k.a. 2610306G12Rik, AW413156)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SacII digestion and SP6 RNA polymerase for antisense probe