pISH-Pou2f1
(Plasmid
#74355)
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PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f1. Not for gene expression.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePOU domain, class 2, transcription factor 1
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Alt namePou2f1
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SpeciesM. musculus (mouse)
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Insert Size (bp)868
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GenBank IDNM_198933.3
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Entrez GenePou2f1 (a.k.a. 2810482H01Rik, NF-A1, Oct-1, Oct1, Otf-1, Otf1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NcoI digestion and SP6 RNA polymerase for antisense probe
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Pou2f1 was a gift from Peter Mombaerts (Addgene plasmid # 74355 ; http://n2t.net/addgene:74355 ; RRID:Addgene_74355) -
For your References section:
Expression of homeobox genes in the mouse olfactory epithelium. Parrilla M, Chang I, Degl'Innocenti A, Omura M. J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19. 10.1002/cne.24051 PubMed 27243442