pISH-Cbr2
(Plasmid
#74339)
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PurposeISH probe for Cbr2
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBlueScript II SK
- Backbone size w/o insert (bp) 2961
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecarbonyl reductase 2
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Alt nameCbr2
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SpeciesM. musculus (mouse)
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Insert Size (bp)462
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GenBank IDNM_007621.2
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Entrez GeneCbr2 (a.k.a. MLCR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Cbr2 was a gift from Peter Mombaerts (Addgene plasmid # 74339 ; http://n2t.net/addgene:74339 ; RRID:Addgene_74339) -
For your References section:
Protocols for two- and three-color fluorescent RNA in situ hybridization of the main and accessory olfactory epithelia in mouse. Ishii T, Omura M, Mombaerts P. J Neurocytol. 2004 Dec;33(6):657-69. Epub 2005 Oct 11. 10.1007/s11068-005-3334-y PubMed 16217621