pISH-Ascl1
(Plasmid #74338)

• Purpose: Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.
• Depositing Lab: Peter Mombaerts
• Publication: Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 1.

Backbone

• Vector backbone: pGEM7Zf
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: achaete-scute family bHLH transcription factor 1
• Alt name: Ascl1
• Alt name: Mash1
• Species: M. musculus (mouse)
• Insert Size (bp): 2003
• GenBank ID: ENSMUST00000020243
• Entrez Gene: Ascl1 (a.k.a. ASH1, Mash1, bHLHa46)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13_pUC_fwd
• 3′ sequencing primer: M13_pUC_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry