pISH-Lhx2
(Plasmid #74337)

• Purpose: Use to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.
• Depositing Lab: Peter Mombaerts
• Publication: Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 1.

Backbone

• Vector backbone: pBlueScript II SK
• Backbone size w/o insert (bp): 2961

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LIM homeobox protein 2
• Alt name: Lhx2
• Species: M. musculus (mouse)
• Insert Size (bp): 1695
• GenBank ID: NM_010710
• Entrez Gene: Lhx2 (a.k.a. LH2A, Lh-2, Lim2, ap, apterous)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results identified a mismatch in the Lhx2 sequence in this plasmid compared to GenBank reference sequence NM_010710. This difference will not interfere with probe performance neither with probe specificity.