pISH-Gap43
(Plasmid #74336)

• Purpose: Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.
• Depositing Lab: Peter Mombaerts
• Publication: Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 1.

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: growth associated protein 43
• Alt name: Gap43
• Species: M. musculus (mouse)
• Insert Size (bp): 714
• GenBank ID: NM_008083
• Entrez Gene: Gap43 (a.k.a. B-50, Basp2, GAP-43)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SphI digestion and SP6 RNA polymerase for antisense probe