pCR8-Magneto2.0-p2A-mCherry
(Plasmid #74334)

• Purpose: For gateway cloning Magneto2.0-p2A-mCherry into destination vector
• Depositing Lab: Ali Guler
• Publication: Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.

Backbone

• Vector backbone: pCR8
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2817
• Vector type: Gateway vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Magneto2.0
• Alt name: TRPV4 (rat); FTL (human); FTH1(human)
• Species: H. sapiens (human), R. norvegicus (rat)
• Insert Size (bp): 4422
• Mutation: TRPV4: delta 760–871
• Entrez Gene: FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
• Entrez Gene: FTL (a.k.a. FTL1, LFTD, NBIA3)
• Entrez Gene: Trpv4 (a.k.a. Otrpc4, Vroac)
• Promoter: none
• Tags / Fusion Proteins:
  • Flag
  • p2A-mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pCR8-Magneto2.0-p2A-mCherry sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry