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Addgene

pH-tdGFP
(Plasmid #74322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 4150
  • Total vector size (bp) 5622
  • Vector type
    Yeast Expression ; yeast tagging
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pH-tdGFP
  • Alt name
    fluorescent protein derived from sfGFP
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    1460
  • Mutation
    N149Y, Q204H
  • Tag / Fusion Protein
    • none (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH-tdGFP was a gift from Joerg Stelling (Addgene plasmid # 74322 ; http://n2t.net/addgene:74322 ; RRID:Addgene_74322)

  • For your References section:

    Identification and Characterisation of a pH-stable GFP. Roberts TM, Rudolf F, Meyer A, Pellaux R, Whitehead E, Panke S, Held M. Sci Rep. 2016 Jun 21;6:28166. doi: 10.1038/srep28166. 10.1038/srep28166 PubMed 27324986
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