5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
(Plasmid #74317)

• Purpose: A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish
• Depositing Labs: Martin Meyer, Stephen Smith
• Publication: Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Backbone

• Vector backbone: pEGFP-N2

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: synaptophysin
• Species: D. rerio (zebrafish)
• Insert Size (bp): 960
• Promoter: 5UAS
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: ATGGATGTTGCCAACCAGTT
• 3′ sequencing primer: TGGACGAGCTGTACAAGTAA

Gene/Insert 2

• Gene/Insert name: DsRedExpress
• Insert Size (bp): 700
• Promoter: 5UAS

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: ATGGCCTCCTCCGAGGACGT
• 3′ sequencing primer: CACCACCTGTTCCTGTAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry