14UAS PSD95:GFP 5UAS DSRedExpress
(Plasmid #74315)

• Purpose: Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish
• Depositing Labs: Martin Meyer, Stephen Smith
• Publication: Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub 2004 Feb 1.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: clontech/invitrogen
• Backbone size w/o insert (bp): 5
• Total vector size (bp): 8
• Modifications to backbone: CMV replaced by 14 UAS repeats

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: zebrafish PSD95
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2406
• Mutation: Y599H and I793S (please see depositor comment below)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Gene/Insert 2

• Gene/Insert name: DsRedExpress

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.