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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
(Plasmid #74309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5375
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPV4-p2A-ferritin-p2A-mCherry
  • Alt name
    TRPV4 (rat); FTL (human); FTH1(human)
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Insert Size (bp)
    4815
  • Entrez Gene
    Trpv4 (a.k.a. Otrpc4, Vroac)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry was a gift from Ali Guler (Addgene plasmid # 74309 ; http://n2t.net/addgene:74309 ; RRID:Addgene_74309)

  • For your References section:

    Genetically targeted magnetic control of the nervous system. Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann CD, Guler AD. Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265. 10.1038/nn.4265 PubMed 26950006
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