pAAV-CMV-DIO-Magneto2.0-sNRPpA
(Plasmid #74307)

• Purpose: Cre-dependent expression of Magneto2.0 in mammalian cells
• Depositing Lab: Ali Guler
• Publication: Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3812
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Magneto2.0
• Alt name: TRPV4 delta 760–871 (rat); FTL (human); FTH1(human)
• Species: H. sapiens (human), R. norvegicus (rat)
• Insert Size (bp): 3650
• Mutation: TRPV4: delta 760-871
• Entrez Gene: FTL (a.k.a. FTL1, LFTD, NBIA3)
• Entrez Gene: FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
• Entrez Gene: Trpv4 (a.k.a. Otrpc4, Vroac)
• Promoter: CMV (after Cre recombination)
• Tag / Fusion Protein:
  • Flag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: F1ori-R

Resource Information

• Supplemental Documents:
  • pAAV-CMV-Magneto2.0-sNRPpA sequence
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry