pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA
(Plasmid #74306)

• Purpose: Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells
• Depositing Lab: Ali Guler
• Publication: Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3812
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TRPV4
• Species: R. norvegicus (rat)
• Entrez Gene: Trpv4 (a.k.a. Otrpc4, Vroac)
• Promoter: CMV (after cre recombination)
• Tag / Fusion Protein:
  • p2A (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: F1ori-R

Gene/Insert 2

• Gene/Insert name: ferritin (FTL and HTL1)
• Species: H. sapiens (human)
• Entrez Gene: FTL (a.k.a. FTL1, LFTD, NBIA3)
• Entrez Gene: FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
• Tag / Fusion Protein:
  • FLAG

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry