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pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA
(Plasmid #74302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2CG2
  • Backbone manufacturer
    Koichi Kawakami Lab
  • Backbone size w/o insert (bp) 7796
  • Vector type
    zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Magneto2.0
  • Alt name
    TRPV4 (rat); FTL (human); FTH1(human)
  • Alt name
    TRPV4-ferritin
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Mutation
    TRPV4: delta 760-871
  • Entrez Gene
    FTL (a.k.a. FTL1, LFTD, NBIA3)
  • Entrez Gene
    FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
  • Entrez Gene
    Trpv4 (a.k.a. Otrpc4, Vroac)
  • Promoter Neurog1
  • Tags / Fusion Proteins
    • FLAG
    • p2A-mCherry (C terminal on insert)
    • cmcl2::EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA was a gift from Ali Guler (Addgene plasmid # 74302 ; http://n2t.net/addgene:74302 ; RRID:Addgene_74302)

  • For your References section:

    Genetically targeted magnetic control of the nervous system. Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann CD, Guler AD. Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265. 10.1038/nn.4265 PubMed 26950006
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