CΔ3
(Plasmid #74298)

• Purpose: Mammalian expression of treacle (residues 1262-1302) fused to GFP
• Depositing Lab: Rita Shiang
• Publication: Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Backbone

• Vector backbone: pS65T-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4728
• Total vector size (bp): 4769
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: treacle ribosome biogenesis factor 1
• Alt name: Tcof1
• Species: M. musculus (mouse)
• Insert Size (bp): 41
• Mutation: Expresses residues 1262 - 1302
• Entrez Gene: Tcof1 (a.k.a. treacle)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ecl136II (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: GFP-F
• 3′ sequencing primer: pEGFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene Sanger sequencing determined that the treacle translation contains residue 1261.